The Ugo Basile 14900 Superfusion System is a semi-automated device to study the release of neurotransmitters in synaptosome samples. It is a development of the instrument originally developed in Prof. Raiteri's laboratory, where about 200 papers have been published exploiting the technique.
The 14900 Superfusion System consists of 12 parallel open superfusion chambers with 12 upper reservoirs, all thermo-stated by a water-jacket. Pre-warmed oxygenated media of the desired composition can be concomitantly delivered from the reservoirs to the superfusion chambers.
Synaptosomes are accomodated as very thin layers on microporous filters placed on glass filter supports. Synaptosome or slice super-fusion is provided by a multi-channel peristaltic pump and superfusate samples are directly collected into scintillation vials.